GeneMarker® HID Human Identity Software: User-Friendly Expert System for Autosomal STR Typing of Forensic DNA Samples
Forensic DNA typing expert systems software (as described in Appendix B NDIS) offers automated sizing and analysis of electrophoretic output, reducing the time required to accurately designate the profile, expedites the process of DNA analysis and reduces casework backlogs; flagging peaks to alert the analyst when defined criteria are not met. GeneMarker HID human identity software is concordant with GeneMapper® ID/GeneMapper® ID-X with many unique features that decrease analysis time and analyst fatigue including; accurate size calling and pattern recognition technology, three easy-to-use dialog boxes to set parameters for consistent analysis and auto-run, customizable viewing modes, linked navigation, automatic intensity-scaling, quality flagging and audit trail, GeneMarker HID software is compatible with all multiplexes (including AmpFSTR®Identiflier™, Minifiler™, GlobalFiler™, Yfiler™, PowerPlex®16, PowerPlex® Fusion, PowerPlex®ESI, PowerPlex®Y) and genetic analyzers (ABI®PRISM 310-3100-3730-3500 HID Genetic Analyzers). Administrative controls allow customization of work-flow and reports (CODIS format, Allele Reports to import into LIMS; Missing Persons, Crime Scene or Familial statistics results).
Three Simple Clicks to Analysis
Setting analysis parameters is accomplished through 3 easy-to-use dialog boxes. Once parameters have been set, they can be saved for future analysis, allowing “single click” operation of the AutoRun feature. Analysis settings include options to correct common chemistry artifacts (Pull-up, Peak Saturation, Spike Removal), selecting positive control template and auto-panel adjust and select best ladder for run-to-run and capillary-to-capillary variations.
The main window of GeneMarker HID has an easy to use layout. The sample files are displayed on the left, the Synthetic Gel Image is displayed at the top, Electropherograms appear below the gel image, and the Report Table is on the right side of the window. To open and close the frames – for example to close the synthetic gel image - use the Show/Hide icons in the main toolbar.
GeneMarker HID has linked navigation; use the mouse to click on a peak to highlight the details in the peak table below the electropherogram and in the allele report at the right. Quality flagging reasons are automatically entered in the table. All edits and comments are also saved in the file history.
Easily Zoom in or out using the mouse to draw a box around the region of interest or double click on the cell of the report table to display the marker. Stutter filters are customized for each marker in the panel editor.
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Complete Human Identity Analysis in one program:
- Accurate, user-friendly expert system genotyping
- Mixture Analysis Application
◦ Immediate ID of mixtures
◦ Automates repetitive calculations
◦ Discriminates major/minor contributors
– with or without a reference sample
- Database Search with stats calculations IBD
◦ Missing Persons
◦ Mass Disaster / UHR
◦ Familial Search
- Kinship Analysis stats (three generations) IBD
◦ Likelihood Ratios and Probabilities
◦ Immigration/Grandparentage/Sib-ships
- Paternity Analysis
◦ Automated Pedigree Drawing
◦ Instant ID of Allele Conflicts (exclusions)
◦ Allow for mutation (AABBmutation rates)
◦ Two options for statistics
– Identity by Descent
– Trio Calculations and motherless cases
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Tools to Assist Analysts and Supervisors:
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