SoftGenetics - Software PowerTools for Genetic Analysis
 
       

 

Forensic STR Profile Analysis Essentials: Panel Editor Tools and Quality Size Call Verification

Panel files (including Identifiler®, Minifiler®, PowerPlex®16, PowerPlex®ESI/ESX) and size standards (including GeneScan™500-GS600 and Internal Lane Standard ILS500-ILS600) are pre-loaded in GeneMarker® HID software; previously saved AB panels can also be imported from GeneMapper®, GeneMapper® ID and GeneMapper® ID-X software; or easily construct a panel for analysis with custom chemistries. Filtering parameters of each locus can be customized in the panel editor, including: Minimum Heterozygous and Homozygous RFUs for Analytical Range and Stochastic Ranges, and percentages for Heterozygous Imbalance and Stutter. Stutter values may be set per the marker or as allele specific stutter (View ISHI 2016 poster). Calibration of panels is a single step performed during validation and enables the auto-panel adjustment for all subsequent analyses. Size call quality is confirmed with linear plots for each file and quality statistics for disabled fragments, as is recommended for GeneScan™500LIZ®, and options to save or print the Sizing Quality Report. GeneMarker HID software imports files from all ABI®PRISM-310-3100-3730-3500 HID genetic analyzers and .fsa, .hid, .scf, .sg1 file formats.

 

 

Customize Panels: Each marker can be edited for minimum calling RFU (analytical range), Inconclusive (stochastic range) Heterozygous Imbalance and Stutter percentages.

 

When an allele ladder sample migration is beyond the auto-panel adjust range the out of bin peaks are flagged with red allele label and red vertical bars in Main Analysis Screen. The figure above from the Panel Editor shows that there may be problems with this capillary,

 

 

Confirm Sizing Quality at a glance: Quality Scores, Electropherograms, Linear Plots and Quality Statistics for disabled fragment(s). Use this screen as a review tool or print/save it for inclusion with the project audit trail.

 

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