SoftGenetics - Software PowerTools for Genetic Analysis
 
       

 

SNPlex® & SNPWave® Analysis

One high-throughput method to determine SNP genotypes is SNPWave (Keygene N.V.), which uses multiplex oligonucleotide ligation amplification of allele-specific probes coupled with AFLP®-primer selective amplification. Basic principle of SNPWave Multiplex allele-discrimination based on ligation using probes with unique stuffer (green). Circularizing padlock ligation probes are constructed that are specific to the SNP and flanking sequences. Locus-specific probes hybridize to denatured genomic DNA. Allele‐specificity is determined by the SNP at the 5' end of the padlock-probe. Probes that contain a 5' nucleotide complementary to the SNP will be ligated and amplified by PCR in subsequent reactions. Alternatively, SNPlex Genotyping (Applied Biosystems) has been used to investigate SNPs in a diverse research, including breast cancer and to genotype plants. GeneMarker software genotyping software is designed for fast, accurate and efficient analysis of SNPlex and SNPWave data from MegaBACE®, Beckman-Coulter® or ABI®PRISM capillary electrophoresis systems.

 

 

GeneMarker software's SNPWave Report

 

Download SNPlex Application Note
Download SNPWave Application Note

 

Trademarks property of their respective owners
Research use only (RUO)

SoftGenetics - Software PowerTools for Genetic Analysis

Software

 

 

 

 

 

 

 


 

 

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

 

 

SoftGenetics - Software PowerTools for Genetic Analysis
HOME | CONTACT US

Please feel free to contact us, representatives are here to help.

 

 

© 2016 SoftGenetics, LLC. All rights reserved


100 Oakwood Avenue
Suite 350
State College, PA 16803
USA


Phone: 814-237-9340 or 888-791-1270
Email: info@softgenetics.com

 

Web design by Lovett Creations