SoftGenetics - Software PowerTools for Genetic Analysis

 

 

 

 

LINKED MIXTURE ANALYSIS FUNCTION & SEARCHABLE DATABASE

Short Tandem Repeat (STR) genotyping is instrumental in data analysis for a wide range of human identification applications. Forensic samples from mass disasters and crime scene investigations are mixtures rather than single source, which require additional analysis after obtaining DNA profiles (per SWGDAM Guidelines). GeneMarker®HID software performs consistent and accurate genotype determination, is compatible with commercial forensic chemistries and major CE genetic-analyzers (including ABI®PRISM, Applied Biosystems SeqStudio™, Promega Spectrum Compact CE System, or a rapid system such as RapidHIT™ and RapidHIT™ID). The embedded mixture analysis application distinguishes between mixtures of 2-contributors and more complex mixtures, automates repetitive calculations (Mixture-Ratios, Likelihood-ratios LRs, Probability-of-Inclusion, Probability-of-Exclusion, Random-Man-Not-Excluded), ranks the most likely genotype combinations (with or without single source reference samples) deducing contributor genotypes, and searches a database for a matching profile or potential relatives. The mixture analysis application uses allele frequency tables from USA populations, or easily imports other population allele frequencies to calculate LRs. This module was developed using recommendations of the DNA Commission of the International Society of Forensic Genetics (Gill et al., 2006) and methods of Clayton et al., 1998 and Gill et al., 1999.

 

The Mixture Analysis Application is directly linked to the main analysis screen - avoiding the error-prone, human transfer of data from one software package to another. Repetitive calculations are automated, according to user-set analysis parameters.

 

View the GeneMarkerHID Mixture Analysis Webinar

 

Click here if interested in Probabilistic Mixture Analysis, MaSTR™ software

 

See figures 1 – 4 (below) for an overview of the benefits of GeneMarkerHID mixture analysis tools

 

Features of the Mixture Analysis Application include:

  • Easy customization of analysis settings to meet the laboratory SOP
  • Groups files by number of contributors
  • Identifies contributor single source files
  • Displays all possible allele combinations for contributors, color-coding those that meet all mixture analysis parameters
  • Determines major and minor contributors
  • Searches database for exact match or potential near relatives

Performs Repetitive Calculations:

  • Major Mx ratio, Residual, Heterozygous Imbalance for each combination of alleles in two contributor mixtures
  • Likelihood Ratios at each marker and combined LR in two contributor mixtures
  • Probability of Inclusion and Probability of Exclusion for any mixture sample (two contributors or three or more)

Potential Mixture Samples are Identified Automatically in Main Analysis Screen

Figure 1: Main analysis screen showing flags for potential mixture samples

 

Identify Potential Suspect without Suspect Reference Profile

Often, a suspect profile is not available. The mixture application deconvolutes the mixture. The report below displays the potential major contributor, which can be used to search laboratory, LDIS, SDIS or CODIS databases. All results are presented in report tables that may be saved or copy/pasted into existing documents.

 

Figure 2: The Mixture Analysis application screen shows that mixtures can be deconvoluted and the LR calculated, even without a reference profile from a suspect

 

Search database using major contributor potential suspect from deconvolution of mixture sample

Figure 3: Click on the Search Database icon to locate matches to potential suspect profiles and calculate the mixture statistics.

 

Mixture Analysis with Two Single Source Reference Files

Figure 4: The Report table has an option to display the trace files of the mixture and all single source profiles that are contained with the mixture allele calls.

SoftGenetics - Software PowerTools for Genetic Analysis

Features and Benefits

Time Saving Linked Tools

Quality Evaluation Tools

Site Tools

 

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

SOFTWARE


Review all of our software products

 

 

 

 

 

 

 

 

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

 

SoftGenetics Software PowerTools for Genetic Analysis provides current up-to-date information and pricing on all products.
All requests are secure.

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

EXPERTISE


Request trial versions of our software and tap into our knowledge base

 

 

Trial Software

All of our software products are available for review and trial periods. Please use link to request a trial version.

 

Request Trial >>

 

Online Training

Our software professionals are available to you for online training of any of our products. Please contact an expert now.

 

Request Training >>

 

Webinars

SoftGenetics provides online webinars to assist you in the growing knowledge of our products. Visit our Analysis Corner.

 

View Webinars >>

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

SEND US A MESSAGE


Representatives are always ready to assist you


Name *
Email *
Phone

###
-
###
-
####
Message *

 

SoftGenetics - Software PowerTools for Genetic Analysis

100 Oakwood Avenue | Suite 350 | State College, PA 16803 | USA
Phone: 814-237-9340 or 888-791-1270
Email: info@softgenetics.com

 

© 2020 SoftGenetics, LLC. All rights reserved

Trademarks are property of their respective owners

Research use only (RUO)

 

Web design by Lovett Creations