SoftGenetics - Software PowerTools for Genetic Analysis


SNPlex® & SNPWave® Analysis

One high-throughput method to determine SNP genotypes is SNPWave (Keygene N.V.), which uses multiplex oligonucleotide ligation amplification of allele-specific probes coupled with AFLP®-primer selective amplification. Basic principle of SNPWave Multiplex allele-discrimination based on ligation using probes with unique stuffer (green). Circularizing padlock ligation probes are constructed that are specific to the SNP and flanking sequences. Locus-specific probes hybridize to denatured genomic DNA. Allele‐specificity is determined by the SNP at the 5' end of the padlock-probe. Probes that contain a 5' nucleotide complementary to the SNP will be ligated and amplified by PCR in subsequent reactions. Alternatively, SNPlex Genotyping (Applied Biosystems) has been used to investigate SNPs in a diverse research, including breast cancer and to genotype plants. GeneMarker software genotyping software is designed for fast, accurate and efficient analysis of SNPlex and SNPWave data from MegaBACE®, Beckman-Coulter® or ABI®PRISM capillary electrophoresis systems.



GeneMarker software's SNPWave Report


Download SNPlex Application Note
Download SNPWave Application Note


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SoftGenetics - Software PowerTools for Genetic Analysis












SoftGenetics - Software PowerTools for Genetic Analysis





SoftGenetics - Software PowerTools for Genetic Analysis

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