|
MS-MLPA®
Methylation-Specific-Multiplex Ligation-Dependent
Probe Amplification for Genetic
Disease and Genomic Imprinting Research
GeneMarker® DNA analysis software
is compatible with all major CE genetic-analyzers,
and is an excellent alternative to
Applied BioSystems GeneMapper® software
or MRC Holland's Coffalyser.Net software.
GeneMarker software is also compatible
with outputs from all major sequencing
systems, including ABI®PRISM, Applied
Biosystems® SeqStudio, and Spectrum
Compact CE genetic analyzers, as well
as custom primers or commercially
available chemistries from MRC Holland.
Detection of methylated sites
in genomic DNA is important
for the following reasons:
- If the promoter is methylated,
the gene will not be transcribed
and its associated protein will
not be produced.
- DNA methylation is important in
genomic imprinting (Prader-Willi
Syndrome and Angelman
Syndrome).
GeneMarker software's Methylation
Specific - MLPA® module quickly and
accurately detects methylation sites
for researchers studying promoter
methylation and genomic imprinting
diseases. GeneMarker software's ease
of use and professional reporting
options make it an excellent choice
for MS-MLPA applications. Panels
for analysis of data from Promoter
Methylation kits from MRC-Holland
(including Tumor Suppressor, Mismatch
Repair genes and genomic imprinting
kits) are included with GeneMarker
software, and are easily downloaded
or available from tech_support@softgenetics.com.
Embedded MS-MLPA
Analysis Module
Figure
1: Methylation of the promoter
region of tumor suppressor gene ESR1
is indicated by the red plot point
that appears above the methylation
threshold line
Comparison
of Normal Methylation vs Unmethylated
Sample
Figure 2:
The image on the left is a normal
sample. Five genes of interest (methylation
specific) from the PWS/AS region of
chromosome 15 appear in the “Normal”
region between the methylation detection
threshold lines. The image on the
right is a sample from a PWS sample.
The five genes of interest are in
a 1:1 ratio with the reference trace
indicating methylation at these five
sites (one copy of 15q11.2 and one
copy of 15q12 have been deleted).
Download
MS-MLPA Application Note (PDF)
MLPA is registered trademark of MRC
Holland
|
