MS-MLPA®
Methylation-Specific-Multiplex Ligation-Dependent
Probe Amplification for Genetic
Disease and Genomic Imprinting Research
GeneMarker® software DNA analysis
software is compatible with all major
CE genetic-analyzers, and is an excellent
alternative to Coffalyser.Net, GeneMapper®,
Genotyper™, GeneScan®,
and CEQ™
Fragment Analysis Software for
MLPA®
for detecting genetic deletions and
duplications associated with various
diseases and cancers. Detection of
methylated sites in genomic DNA is
also important: 1) If the promoter
is methylated, the gene will not be
transcribed and its associated protein
will not be produced. 2) DNA methylation
is important in genomic imprinting
(Prader-Willi
Syndrome and Angelman
Syndrome). Panels
for analysis of data from Promoter
Methylation kits from MRC‐Holland
(including Tumor Suppressor, Mismatch
Repair genes and genomic imprinting
kits) are included with GeneMarker
software, and are easily downloaded
or available from tech_support@softgenetics.com.
GeneMarker software's Methylation
Specific - MLPA® module quickly
and accurately detects methylation
sites for researchers studying promoter
methylation and genomic imprinting
diseases. GeneMarker software's ease
of use and professional reporting
options are an excellent choice for
MS‐MLPA® applications.
Methylation of the promoter region
of tumor suppressor gene ESR1 is indicated
by the red plot point that appears
above the methylation threshold line:
The image on the left is a normal
sample. Five genes of interest (methylation
specific) from the PWS/AS region of
chromosome 15 appear in the “Normal”
region between the methylation detection
threshold lines. The image on the
right is a sample from a PWS sample.
The five genes of interest are in
a 1:1 ratio with the reference trace
indicating methylation at these five
sites (one copy of 15q11.2 and one
copy of 15q12 have been deleted).
Download
MS-MLPA Application Note (PDF)
MLPA is a registered trademark of
MRC-Holland B.V.
Trademarks property
of their respective owners
Research use only (RUO)
