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Microsatellite
Instability (MSI) Analysis Comparing
Normal and Tumor Samples
MSI analysis is essential for tumor
research, such as colorectal,
triple negative breast,
EMAST
(Elevated Microsatellite instability
at Selected Tetranucleotide repeats)
and bladder
cancer. This instability is due
to either insertion or deletion of
repeating units during DNA replication
and failure of the mismatch repair
system (MMR) to correct these errors.
The MSI Application
in GeneMarker® software is directly
linked to the main analysis screen
and is an excellent tool for analysis
from kits such as the Promega
MSI Analysis System, or Applied
Biosystems MSI Primers separated
with ABI PRISM® , SeqStudio™ or Spectrum
Compact CE Systems genetic analyzers.
GeneMarker software's MSI Analysis
module compares tumor to normal
samples based on peak-to-peak comparison
to differentiate between MSH-H
and MSS samples. See
figures 1 and 2 below for an overview
of the MSI application.
Download
the application note.
MSI Normal
and Tumor Electropherogram Overlay
View
Figure
1: The tumor electropherogram
for each sample is displayed in an
overlay with the normal electropherogram
(red trace) for easy visualization
of any instability.
Customized
Reporting
Figure
2: Includes a header for
electronic signature, trace overlay
(tumor and normal), trace comparison
histogram and gain/loss table.
MLPA® is the registered trademark of MRC Holland
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