SoftGenetics - Software PowerTools for Genetic Analysis

 

 

 

 

 

Whole Genome Alignment

SoftGenetics has developed for NextGENe software a modified Burrows-Wheeler transform (BWT) alignment method that includes several improvements over other methods to generate fast accurate alignment of sequence reads to a whole large genome reference such as the human genome with high accuracy and speed.


NextGENe software's whole genome alignment algorithm is a multi-step process that first aligns perfectly matching reads, then reads with a user set number of mis-matches and finally it breaks the remaining reads into seeds. The algorithm then aligns reads to a large genome by matching seeds smaller than the read length and then extending the alignment to find the best matching position for the whole read. This allows for the alignment of long reads and reads with indels.


The NextGENe whole genome alignment method uses an indexed reference sequence. Indexed references with full annotation are available for many genomes including the human genome, mouse genome, and many more. A tool for building custom indexed reference is also included. Alignment of high throughput short sequence reads to a large reference genome like the human genome is a difficult challenge.


The Burrows-Wheeler transform is a widely accepted data compression algorithm that has been in use since 1994. Massively parallel sequencers such as Illumina® iSeq, Miniseq, MiSeq, NextSeq, HiSeq, and NovaSeq systems, Ion Torrent Ion GeneStudio S5, PGM, and Proton systems as well as other platforms are capable of producing millions or billions of reads per run, which has led to an interest in the usage of BWT algorithm to align this large volume of sample reads to entire genomes. The algorithm has been successfully used for this purpose by alignment programs such as Bowtie and BWA.

 

For More Information Review or Download Application notes:
Whole Genome Alignment with NextGENe software
NextGENe Illumina SNP_Indel_Detection

NextGENe software Variant Comparison Tool
Deep Sequence Analysis with NextGENe software
Whole Genome Alignment with NextGENe software
Sequence Capture Analysis with NextGENe software
Using NextGENe software's Pipeline Automation Tool

 

Webinar:

Creating Sequence Alignment Projects with NextGENe (View on )

 

Pricing & Trial Version:
Request 30-day trial/Price Quote

 

Reference Material:
Download/View NextGENe® Brochure
NextGENe User Manual
Annotated Reference Genomes

 

Trademarks property of their respective owners

SoftGenetics - Software PowerTools for Genetic Analysis

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

SOFTWARE


Review all of our software products

 

 

 

 

 

 

 

 

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

 

SoftGenetics Software PowerTools for Genetic Analysis provides current up-to-date information and pricing on all products.
All requests are secure.

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

EXPERTISE


Request trial versions of our software and tap into our knowledge base

 

 

Trial Software

All of our software products are available for review and trial periods. Please use link to request a trial version.

 

Request Trial >>

 

Online Training

Our software professionals are available to you for online training of any of our products. Please contact an expert now.

 

Request Training >>

 

Webinars

SoftGenetics provides online webinars to assist you in the growing knowledge of our products. Visit our Analysis Corner.

 

View Webinars >>

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

SEND US A MESSAGE


Representatives are always ready to assist you


Name *
Email *
Phone

###
-
###
-
####
Message *

 

SoftGenetics - Software PowerTools for Genetic Analysis

100 Oakwood Avenue | Suite 350 | State College, PA 16803 | USA
Phone: 814-237-9340 or 888-791-1270
Email: info@softgenetics.com

 

© 2020 SoftGenetics, LLC. All rights reserved

Trademarks are property of their respective owners

Research use only (RUO)

 

Web design by Lovett Creations