ThunderBolts™
Panel Analysis
A major challenge facing today's
cancer researchers is the ability
to have highly accurate, simple, and
low-cost tools to rapidly identify
genetic variants underlying specific
phenotypes found in solid tumors and
hematological cancers. The combination
of the RainDance ThunderBolts™
System, the ThunderBolts NGS gene
panels, and SoftGenetics NextGENe®
Software enables targeted sequencing
for research of important oncogenes
that have clinical relevance to cancer
diseases. The solution features preloaded
analysis templates for each gene panel
and pre-defined settings for detecting
minor allele frequencies at different
sensitivity settings (1% and 5%).
NextGENe version 2.4.1 introduces
AutoRun Templates for quick project
setup using various types of library
preparations. The ThunderBolts Cancer
Panels and ThunderBolts Myeloid Panels
are installed by default. They can
be loaded into the NextGENe AutoRun
tool to quickly set up a job to process
multiple samples. For each panel,
NextGENe includes a template with
5% allele frequency cutoff and a "High
Sensitivity" template with 1%
allele frequency cutoff. These pre-defined
templates cannot be modified, but
they may be used to create new custom
templates by users with adequate permissions.
These templates help to ensure that
technicians are using the appropriate
settings and automatically creating
the necessary reports. After processing,
researchers can view projects in the
NextGENe Viewer and (optionally) export
to Geneticist Assistant™.
Panel templates offer an easier approach
for targeted resequencing data in
NextGENe. Templates are used to specify
all settings for the analysis when
creating ngjob files. These ngjob
files are detected and processed by
the AutoRun tool, when sequencing
data is available.

The NextGENe AutoRun Tool’s
Job File Editor includes built-in
templates for ThunderBolts panels.

NextGENe Viewer display with
Mutation Report listing detected variants

The Coverage Curve Target
Region Statistics reports coverage
information such as % of reads on
target, minimum, maximum and average
coverage across all targets, and the
percentage of targets covered at a
user-set coverage level.
For further information please
review or download the following Application
Note:
NextGENe®
Software Analysis of Solid Tumors
and Hematological Cancers Using RainDance
ThunderBolts™ NGS Panels
Webinar:
Analysis of Raindance Thunderbolts
Panel Data with NextGENe Software
(View)
(view
on )
Pricing & Trial Version:
Request
30-day trial/Price Quote
Reference Material:
Download/View
NextGENe® Brochure
NextGENe
User Manual
Annotated
Reference Genomes
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