SoftGenetics - Software PowerTools for Genetic Analysis

ThunderBolts™ Panel Analysis

A major challenge facing today's cancer researchers is the ability to have highly accurate, simple, and low-cost tools to rapidly identify genetic variants underlying specific phenotypes found in solid tumors and hematological cancers. The combination of the RainDance ThunderBolts™ System, the ThunderBolts NGS gene panels, and SoftGenetics NextGENe® Software enables targeted sequencing for research of important oncogenes that have clinical relevance to cancer diseases. The solution features preloaded analysis templates for each gene panel and pre-defined settings for detecting minor allele frequencies at different sensitivity settings (1% and 5%).

NextGENe version 2.4.1 introduces AutoRun Templates for quick project setup using various types of library preparations. The ThunderBolts Cancer Panels and ThunderBolts Myeloid Panels are installed by default. They can be loaded into the NextGENe AutoRun tool to quickly set up a job to process multiple samples. For each panel, NextGENe includes a template with 5% allele frequency cutoff and a "High Sensitivity" template with 1% allele frequency cutoff. These pre-defined templates cannot be modified, but they may be used to create new custom templates by users with adequate permissions. These templates help to ensure that technicians are using the appropriate settings and automatically creating the necessary reports. After processing, researchers can view projects in the NextGENe Viewer and (optionally) export to Geneticist Assistant™.

Panel templates offer an easier approach for targeted resequencing data in NextGENe. Templates are used to specify all settings for the analysis when creating ngjob files. These ngjob files are detected and processed by the AutoRun tool, when sequencing data is available.

The NextGENe AutoRun Tool’s Job File Editor includes built-in templates for ThunderBolts panels.

NextGENe Viewer display with Mutation Report listing detected variants

The Coverage Curve Target Region Statistics reports coverage information such as % of reads on target, minimum, maximum and average coverage across all targets, and the percentage of targets covered at a user-set coverage level.

For further information please review or download the following Application Note:
NextGENe® Software Analysis of Solid Tumors and Hematological Cancers Using RainDance ThunderBolts™ NGS Panels

Webinar:
Analysis of Raindance Thunderbolts Panel Data with NextGENe Software (View) (view on )