SoftGenetics - Software PowerTools for Genetic Analysis


Automated Batch Processing of Sanger Sequencing Data

Mutation Surveyor's AutoRun Tool allows users to quickly carry out the batch analysis of multiple Sanger sequencing projects, including direct sequencing, PCR sequencing, Mitochondrial DNA sequencing, and resequencing projects. The automated batch processing feature enables users to create projects to be processed at specified time intervals, taking advantage of Mutation Surveyor's superior sensitivity and accuracy in the detection of single nucleotide polymorphisms (SNPs), insertion and deletions (INDELS), and low frequency variants (somatic, mosaic, heteroplasmic). Job Files are created to specify project settings, sample files, reference files, and post-analysis reports. Mutation Surveyor AutoRun then scans a directory for job files, and when files are available, the AutoRun tool automatically launches Mutation Surveyor to complete the analysis. Job files may also contain sample files that have not yet been generated by a capillary electrophoresis instrument. The AutoRun function can then be configured to process the job at a time interval after the files are added to the directory.



Select Project and Report Settings in Log File Wizard



The Log File Wizard guides the user through the creation of a log file. The wizard enables users to upload project sample files, reference files, analysis settings files, and report settings into a project. Multiple projects can be included per log file, each containing specific settings and output directories selected by the user. After creating log files, the AutoRun tool is set to scan a directory at a specified time for new log files. The tool then processes unread log files at user-set time intervals, automatically compiling mutation projects and reports and depositing them in the final directory for review.


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