To arrange paired reads
You use the Arrange paired reads option to arrange the reads in your sample files before you carry out sequence alignment. (NextGENe skips the step of arranging the sample files when you load the arranged files as the input files in the Project Wizard. See
Sequence Alignment Project Output Files.1. In the Input pane, click Add to browse to and select the paired read files that are to be arranged.
2. In the Output field, you can leave the default value for the location of the output files as is (the default value is the directory path for the input files), or you can click Set to select a different location.
3. Optionally, before you process the files, click Save to save the settings that you have specified to a Settings file (.ini file).
| You can always load this file at a later date and process other data files according to the saved settings in the file. |
4. Click OK.
A message opens when the process is completed. Two output files that contain the arranged reads are created, for example, sampleA_1_arranged.fasta and sampleA_2_arranged.fasta.