NextGENe Online Help : NextGENe Tools : The NextGENe Sequence Operation Tool : To remove duplicate reads
To remove duplicate reads
Two algorithms are available for removing duplicate reads:
• Algorithm 1 uses the nucleotide sequence. See The Nucleotide Sequence algorithm for removing duplicate reads.
• Algorithm 2 uses an index file that relates to Unique Molecular Identifiers (UMIs). See The UMI algorithm for removing duplicate reads.