To reverse complement sequences
1. In the Input pane, click Add to browse to and select the .fasta file or .fastq file for which the sequence reads are being reverse complemented.
2. In the Output field, you can leave the default value for the location of the output files as is (the default value is the directory path for the input file), or you can click Set to select a different location.
3. Optionally, before you process the files, click Save to save the settings that you have specified to a Settings file (.ini file).
 | You can always load this file at a later date and process other data files according to the saved settings in the file. |
4. Click OK
A message opens when the process is completed. A single file is produced and its name is appended with the phrase “_complemented” as shown in the figure below.
