NextGENe Online Help : NextGENe Tools : The NextGENe Condensation Results Filter Tool
The NextGENe Condensation Results Filter Tool
You use the Condensation Results Filter tool to filter contaminants such as foreign DNA or primers from condensation reads or assembly results. The filtering is based on different characteristics of condensed reads or assembled contigs. You can remove primer contamination by selecting the Filter by Coverage option to remove very high coverage regions. If foreign DNA contamination is a concern, you can use the Reads Simulator Tool to break the genome and reassemble it with condensed reads. In this case, the option to Filter by Length removes contamination as reads that are assembled with the genome are likely contaminants. You use an Index Error Correction option for transcriptome analysis, where expression levels vary greatly. This option allows indices that differ by only a one base, but that have matching shoulder sequences, to be indexed together when the ratio of the frequency of the minor index to the frequency of the whole group falls below a set threshold.