NextGENe Online Help : NextGENe Tools : The NextGENe GC Percentage Calculation Tool : To use the NextGENe GC Percentage Calculation tool
To use the NextGENe GC Percentage Calculation tool
1. On the NextGENe main menu, click Tools > GC Percentage Calculation.
The GC Percentage Calculation dialog box opens.
2. In the Load File pane, click Set to browse to and select the input file for which the GC content is being calculated.
3. In Output GC Percentage File pane, click Set to specify the name of the output file and the location of the output file.
4. Click OK.
The output file is saved as a .txt file. It lists the GC content every 31 bp for the sample data file.