Setting | Description |
|---|---|
Load Assembled Results File | The Assembly tool creates the assembledsequences.fasta file, which is a file that contains information about each read that was used to create a given assembled contig. You can load this file into the Sequence Alignment tool for a more accurate representation of coverage. Note: For SOLiD System data, you can load the assembledsequences.csfasta file. |
Load Paired Reads | Select this option to align paired-end/mate-paired data sets. |
• Library Size | • The length of the DNA fragment that is used for sequencing pairs. |
• 454 Sequences | • Enter the known sequence separating pairs for Roche/454 paired end analyses in this field. |