Table Field | Default | Description |
---|---|---|
Category | One of three categories for the marker: Amelogenin, Autosomal STR, or ChrY STR. | |
Marker | X | The autosomal STR or Y-STR locus (marker) name. |
Allele Name | X | The capillary electrophoresis (CE) name for a marker. |
Report | X | Selected by default. If selected, the allele sequence is included in the NGS and CE reports. See STR Analysis viewer reports. |
Filter Status | X | One of two statuses is possible based on the STR Filter settings and STR Stutter Filter settings that are specified for the project. • Passed: A read passed all specified STR settings. • Flagged: A read failed one or more specified STR settings. |
Filter Reason | X | The STR filter settings and the STR Stutter filter settings that were triggered if a read was flagged.; otherwise, if a read passed, then the Filter Reason is set to > IT, which indicates that the allele counts were above the interpretative threshold. |
SW Call | X | The software call based on the analysis parameters., where: • Called means all analysis parameters were met. • -1 Stutter (and any other stutter positions) are based on the following calculation: Sequence Total Count of potential stutter peak/Allele Total Count of true peak = Peak height ratio of potential stutter peak to True allele If the value of this calculation falls below the STR Stutter filter settings, then the call is Stutter; otherwise, if the value falls above the STR Stutter filter settings, then the call is a True Peak. Note: If multiple stutter calls are available for an allele, then the SW call differentiates between them. |
User Call | X | You can double-click the User Call for a flagged Filter Status to open the Edit User Call dialog box and enter a custom user call. See To work with user calls and comments in the STR Results Table pane. |
User Comment | X | Displays any optional user comments that were made in support of/explanation of the User Call. See To work with user calls and comments in the STR Results Table pane. |
Bracket Sequence | X | The STR repeat sequence for the allele. The specified Bracket Sequence settings determine the format for the sequence display. See To edit the bracket sequence settings. |
Sequence Group | A grouping method that was used prior to Bracket Sequences. Maintained only for backwards compatibility. | |
Sequence Forward Count | X | The total number of forward reads that contain the bracket sequence. |
Sequence Reverse Count | X | The total number of reverse reads that contain the bracket sequence. |
Sequence Total Count | X | The total number of reads (F+R) that contain the bracket sequence. |
Sequence Allele Percent | X | The percentage of the reads that contain the bracket sequence for the allele, calculated as Sequence Total Count/Allele Total Count. |
Sequence Marker Percent | X | The percentage of the reads that contain the bracket sequence for the marker, calculated as: Sequence Total Count/Marker Total Count. |
Sequence Balance Ratio | X | The greatest value from all the following calculations: • Sequence Forward %/Marker Forward % • Marker Forward %/ Sequence Forward % • Sequence Reverse %/Marker Reverse % • Marker Reverse %/Sequence Reverse % |
Allele Forward Count | X | The total number of reads for the allele in the forward direction, which includes filtered reads and sequence variants that have the same CE allele name. |
Allele Reverse Count | X | The total number of reads for the allele in the reverse direction, which includes filtered reads and sequence variants that have the same CE allele name. |
Allele Total Count | X | The total number of reads for the allele, which includes filtered reads and sequence variants that have the same CE allele name. |
Allele Total Count Filtered | X | The number of reads for the allele that were filtered. |
Allele Marker Percent | X | The ratio of the allele to all alleles for the marker. |
Marker Forward Count | The total number of reads for the marker in the forward direction, including filtered reads. | |
Marker Reverse Count | The total number of reads for the marker in the reverse direction, including filtered reads. | |
Marker Total Count | X | The total number of reads (F+R) for the marker, including filtered reads. |
Marker Total Count Filtered | X | The total number of reads for the marker that were filtered. |
Sequence | X | The complete sequence for the read, with flanking sequences shown in lower case and repeat sequences shown in upper case. |
Left Flank | X | The sequence for the left flank of the sequence read. |
Repeat | X | The sequence for the repeat portion of the reads, but it is not shown in brackets. |
Right Flank | X | The sequence for the right flank of the sequence read. |