To edit the STR Stutter filter settings
When you are editing the STR Stutter filter settings, note the following:
• The default value of All means that which means that all the STR filter settings are applied to all the alleles for all the markers in a project sample.
• You can add more filter settings, delete any filter settings, or both.
• If you add more filter settings, then marker-specific settings and allele-specific setting take precedence over “All” filter settings.
• You can select only a single filter at a time for deletion.
• If you edit any of the default values for the STR Stutter filter settings, and want to return them to their default values, click Default, and then click Yes in the Default values message that opens. The values for all STR Stutter filter settings are immediately reset to their default values.
1. Edit the settings as appropriate for the project.
 | To open a tooltip that provides an explanation of the STR Stutter filter setting, hold your mouse pointer over the column header for the setting. |
Setting | Description |
|---|---|
Marker | To add a marker-specific STR Stutter filter setting: 1. Click Add Filter. A new row is displayed for adding a stutter filter. 2. On the Marker drop-down list, select a marker. 3. Leave the From Allele value and the To Allele Value set to All. |
Repeat Length | The marker repeat length. Automatically populated based on the loaded panel. You should not edit this value. |
From Allele To Allele | The default value for both parameters is All. You must leave these values as-is for marker-specific stutter positions. If allele-specific stutter filters are appropriate, for example, the LUS stutter filter, then do the following: 1. Click Add Filter. A new row is displayed for adding a stutter filter. 2. On the Marker drop-down list, select a marker. 3. Edit the value for the From Allele parameter and then tab out of the field. The value for the To Allele parameter is automatically adjusted. |
Type | Set the stutter position: • -1: One repeat smaller. • -2: Two repeats smaller. • -0.5: 1/2 repeat smaller, typically 2 bp smaller. • +1: One repeat larger. • +0.5: 1/2 repeat larger, typically 2 bp larger. |
Ratio | The ratio of the total number of sequence counts in the stutter position to the total number of allele counts for the main peak. |