The NextGENe Barcode Sorting Tool
If your data files contain barcodes (also referred to as
multiplexed data), then you must use the NextGENe Barcode Sorting tool to
parse the barcoded read data into separate files prior to analysis. NextGENe’s Barcode Sorting tool parses the barcoded sample files into separate files according to sequence tags. You can use the Barcode Sorting tool for data files in which the barcodes are included within the sequence reads, the barcodes are included in the read names or the barcodes are contained in a separate file. Two options are available for trimming the tags from the reads and parsing the reads according to the tags:
• If all the barcode details are known (barcode sequence tags and the sample ID that they represent), you can create a
Barcode/Primer file, which is a tab delimited text file, to provide information to the NextGENe Barcode Sorting tool about the sample IDs, the forward barcode/primer tags, and the reverse barcode/primer tags.
• If some or all the barcode details are not known, you can use the NextGENe Barcode Sorting tool to automatically detect the barcode sequence tags and total tag count and then create separate folders for each tag.