Barcode/Primer File
You can use a program such as Microsoft Excel to create a Barcode Primer File and save the file as a tab-delimited text file. Each line in the file must include the sample ID and an entry for each barcode tag in the sample. The figure below is a sample Barcode/Primer file with just two tags for each sample. Each line in the file includes the sample ID (Sample_ID), the forward barcode tag (Forward Tag) and the reverse barcode tag (Reverse Tag).
Example of a Barcode/Primer file with two tags
 
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If reverse tags are not used, you can leave the Reverse Tag column blank.
The figure below is a sample of a Barcode/Primer file with multiple tags for each sample.