Single Reads report
The Single Reads paired is generated for all single aligned reads. This report provides the name and the position of all reads that aligned to the reference genome without a mate. After you select the Single Reads report option, a Filter Settings dialog box opens. You specify the range for which to generate the report in this dialog box.
Setting | Description |
|---|---|
• Input Region Manually • Entire Reference Range | You must specify the starting position and the ending position, or you can select Entire Reference Range to include the entire reference range in the output. |
Input points of interest text file (*.txt) | There are no special requirements for uploading a comma-delimited text file. If the input text file is a comma-delimited text file, it must contain one of the following lists: • A list of specific reference locations (position number) separated by commas • A list of reference ranges (start position number - end position number) separated by commas |
Input points of interest BED file (*.bed) | A BED file is a tab-delimited text file. You can upload a BED file only if the reference sequence contains chromosome information, which means that the reference sequence must be either a preloaded reference that NextGENe supplies, or a GenBank reference file that contains chromosome information. |
The report is interactive:
Option | Description |
|---|---|
Sort the report results | Double-click any column heading in the report. |
View a position or region in the Alignment viewer | Double-click any value in the report. |
Change the filter settings for the report and dynamically update the report display | 1. On the report menu, click Settings > General Settings. The Filter Settings dialog box opens. 2. Specify the range for which to generate the report, and then click OK to close the dialog box. |
Save the report to a text file | Do either of the following: • On the report menu, click File > Save. • On the report toolbar, click the Save Report icon  .A default name (<project name>_Output_SingleReads) and location (the project output folder) are provided for the file, but you can change one or both of these values. |