Differential gene expression analysis of transcriptome data
 | Before you can use the Expression Comparison report in conjunction with Pathway Studio, your Pathway Studio access option must be set for the NextGENe Viewer. See Process menu (NextGENe Viewer main menu) in NextGENe Viewer main menu. |
 | For instructions about carrying out a detailed differential expression pathway analysis in Pathway Studio, see the following SoftGenetics application note: “http://softgenetics.com/PDF/PathwayStudioAppNote.pdf. |
Differential gene expression generates extraordinarily complex networks of gene and protein interactions. To understand the molecular mechanisms of health and disease, these networks must be identified and understood. You can use the Expression Comparison report to identify the genes that are differentially expressed in transcriptome (RNA-Seq) data, and then upload the report results to Pathway Studio for visualization of the differential expression analysis values as well as biological pathway information. A separate subscription to Pathway Studio is required to access Pathway Studio from NextGENe. See The Expression Comparison Report.