To carry out a secondary analysis
You can use secondary analysis to set up a new project that is based on the output from a previously created project that has yet to be processed. After the previously created project is run, then the secondary analysis of its output files is automatically carried out.
 | You can also carry out a secondary analysis of a previously created project using the NextGENe AutoRun tool. See The NextGENe AutoRun Tool. |
1. Click Create More Projects (Secondary Analysis).
The Project Wizard is opened again.
2. Select the application type for the secondary analysis, and then click Load Data.
The Load Data page opens. The sample files and reference files from the previously created project remain loaded. The page now contains a Load Previous Run Result at the top of the page.
3. Next to the Sample files pane, click Removal All.
All the previously loaded sample files are removed.
4. Click Load Previous Run Result.
The Load Previous Run Result dialog box opens. The availability of what you can select for secondary analysis—Matched reads, Unmatched reads, Pseudo paired reads, Exported reads, and Assembled sequences—is dependent on the settings for the previous run.
| | Typically, Unmatched reads is always available for a secondary analysis. |
5. Select the data type for the secondary analysis.
The Previous run result (Original) list is updated with the appropriate output files from the previous run.
6. Select the appropriate file or files (CTRL-click to select multiple files) in the Previous run result (Original) list, and then click Add to List.
The selected output files are moved to the Previous run result (Added) list.
7. Click OK.
The Load Previous Run Result dialog box closes. You return to the Load Data page in the Project Wizard. The added files are now displayed in the Sample files pane.
8. Modify any settings as needed, and then complete the running of the project in the wizard.