To use the NextGENe Sequence Operation tool
1. On the NextGENe main menu, click Tools > Sequence Operation.
The Sequence Operation dialog box opens.
 | The options that are available on the Sequence Operation dialog box are dynamically updated based on the selected sequence operation. |
2. Do one of the following:
• Select Split files, and then continue to To split files.
• Select Sequence trim, and then continue to To sequence trim reads.
• Select Arrange paired reads, and then continue to To arrange paired reads.
• Select Remove duplicate reads, and then continue to To remove duplicate reads.
• Select Reverse/Complement seq, and then continue to To reverse complement sequences.
• Select Random subsample, and then continue to To create a random subsample.
| | Optionally, instead of manually selecting the settings for any of these operations, you can click Load to browse to and select a Settings file (.ini file) to process the files based on the saved settings in the file. You can click Save after you specify the settings for any of these operations to save the settings to a Settings.ini file. |