MaSTR
Probabilistic Mixture Analysis of STR Profiles

Technology

MaSTR™ software’s unique, easy-to-use interface enables analysts to create and submit mixture analysis jobs in minutes. The server-client configuration provides flexibility and lowers costs by performing the analyses on a dedicated computer, freeing up the analyst’s time and computer for other tasks.

  • Provides a framework for growth:
    - MaSTR software easily adapts future chemistries (Figure 1)
    - Upgrade statistical approach without changing software version
  • Utilizes the laboratory’s unique data set to “train” the software (Figure 2)
  • After validation, lock in models for casework

Models for the current statistical approach are preloaded in MaSTR software; statistical advances will be included with software updates. Researchers can easily experiment with new model variables and submit to the peer reviewed process for publication and advancement of forensic probabilistic analysis.

Interface

Figure 1: Simple, drop-down menus enable labs to update panels with new loci, population allele frequencies, and staff profiles to the elimination database.

Protocol Set

Figure 2: Protocol data sets – replicates of single source dilutions produced following lab SOP enable MaSTR software to calculate:

1. Expected peak height variation at different RFU (statistical profile).
2. Variation from typical degradation of signal due to lower amount of amplified product in higher mw loci.
3. Allelic stutter percentages.

Models

MaSTR software provides flexible technology that allows custom modeling and development.

Figure 3: The easy-to-use interface provides tools that allow forensic scientists to utilize their expertise without scripting. Researchers can easily experiment with new model variables and submit to the peer reviewed process for publication and advancement of forensic probabilistic analysis.