NextGENe® software includes many useful tools for biologists performing targeted resequencing projects containing single nucleotide polymorphism (SNPs) insertions/ deletions (indels) and large structural DNA rearrangements in sequencing data from Illumina® iSeq, Miniseq, MiSeq, NextSeq, HiSeq, and NovaSeq systems, Ion Torrent Ion GeneStudio S5, PGM, and Proton systems as well as other platforms. NextGENe software's Windows® based operation removes the complexity found with programs such as CLC Genomics Workbench, Lasergene's SeqMan Pro, as well as academic software such as MAQ & SOAP, Top Hat, BWA & Bowtie. Biologists simply input the system’s raw reads or BAM files, select the application and click go to begin analysis. Pooled or barcoded samples are quickly de-multiplexed by utilizing the included Barcode Sorting tool, which will separate out each pooled sample for subsequent analysis. NextGENe's Pipeline Tool, AutoRun can be used to set up an unlimited number of consecutive analyses on an unattended basis. Upon completion results are presented in NextGENe software's annotated whole genome browser/viewer for review/editing/commenting.
Specialized resequencing tools include filtering by BED files, Regions of Interest, removal of PCR duplicate reads, and the ability to align to the entire genome or use one or several GenBank (.gb) files.
NextGENe software features greater than 99% accuracy, indel detection up to 33% of read length; rapid review of DNA Variants, nucleotides and Amino Acids; Variant Scoring; Filtering; and analysis comparison. Genome viewer includes links to dbSNP and information from imported tracks, such as dbNSFP, dbSNP, gnomAD and any custom tracks, can be displayed.
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